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Integrated Genomics

Integrated Genomics Facility (IGF)

Lab # 4511, 4732, 4411, 4716 Throckmorton
Plant Sciences Center
Department of Plant Pathology
Kansas State University
Manhattan, KS 66506

phone: (785) 532-1393

Integrated Genomics Facility Equipment Access

Assistance to first-time users of Integrated Genomics Facility equipment and software is available through personal training, or workshops (PLPTH 780, 785).

Equipment at IGF

NanoDropThe NanoDrop spectrophotometer requires only 1-2 µl of a DNA, RNA, protein, and cell culture sample. Its unique feature in monitoring dye incorporation rate after labeling and before microarray hybridization is a very useful function.

NanoDrop website

KASPThe CFX 96 Real-Time PCR System build on Bio-Rad's proven 1000-series thermal cycling platform. The CFX 96 is factory-calibrated, no further calibration will require. It allows up to 5-target multiplexing. The reaction volume can be as low as 10ul. It includes thermal gradient feature. At every position and with every scan, the optics shuttle is reproducibly centered above each well, so the light path is always optimal and there is no need to normalize to a passive reference dye. CFX Manager software can be used not only to visualize all run data at once, but also to perform the complete gene expression analysis.

KASP protocol

Software:

Premier Biosoft

Primer3Plus

Equipment Producer:

BioRad Laboratories

qPCR website:

Gene - Quantification

HydroShearThe HydroShear DNA shearing device is one of the best ways to fragment DNA for genomic and paired end library construction, high  throughput and long reads sequencing. It is ideal for uniform and reproducible making of large DNA fragments (3kb, 8kb, 20kb) for  long reads with next- and 3rd generation sequencers.

DIGILAB HydroShear

Synergy H1 Multi-mode ReaderThe Synergy H1 Multi-mode Reader supports top and bottom fluorescence intensity, UV-visible absorbance and high performance luminescence detection. It is the ideal system for all the standard microplate reader applications found in life science research laboratories.

PicoGreen_Based_Quantification_IGF_Protocol

BioTek

QIAgility Liquid Handling SystemQIAgility Liquid Handling System (QIAGEN) enables rapid, high precision automated transfer of liquids, for as low as 1ul, into almost any tube or plate format, including 384 well plates.

QIAgility (Qiagen)