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Integrated Genomics

Integrated Genomics Facility (IGF)

Lab # 4511, 4732, 4411, 4716 Throckmorton
Plant Sciences Center
Department of Plant Pathology
Kansas State University
Manhattan, KS 66506

phone: (785) 532-1393

1. Illumina's MiSeq Personal Sequencing System

Sample Submission

MiSeq Sequencing System

 

 

 

 

 

 

MiSeq System Performance

 300 cycles kit500 cycles kit600 cycles kit
Read Length2 X 1502 X 2502 X 300
Yield (Gb)~4.5~7.5~14
Run Time (hours)~ 1 day~ 1.5 days~2 days
% > Q30>75>75>70
Clusters/ Run (M)~15~15~23

 

2. Illumina's NextSeq 500 Sequencing System

 NextSeq500


 

 

 

 

 

 

NextSeq500 System Performance

 

High-Output

300 cycles

High-Output

150 cycles

High-Output

75 cycles

 Mid-Output

300 cycles

 Mid-Output

150 cycles

Read Length (bp) 2x150 2x75 1x75 2x150 2x75
Yield (Gb) 100-120 50-60 25-30 32.5-39 16.25-19.5
Run Time (hours) 29 18 11 26 15
% > Q30>75% >80% >80% >75% >80%
Clusters/ Run (M) 400 400 400 130 130

For a sample submission, cost estimate, or additional information please contact Alina Akhunova at akhunova@ksu.edu

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