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Integrated Genomics

Integrated Genomics Facility (IGF)

Lab # 4511, 4732, 4411, 4716 Throckmorton
Plant Sciences Center
Department of Plant Pathology
Kansas State University
Manhattan, KS 66506

phone: (785) 532-1393

DNA, RNA, or Protein quantification with Qubit 2.0 Fluorometer

Qubit 2.0 Fluorometer

Absorbance measurements made on a spectrophotometer includes the  absorbance of all molecules in the sample  that absorb at the wavelength of interest.  Since nucleotides, RNA, ssDNA, and dsDNA  all absorb at the same wave length (260  nm), they will contribute to the total  absorbance of the sample. We highly  recommend using fluorometry for more  accurate DNA, RNA, or Protein  quantification in your projects.

Qubit 2.0 Fluorometer 

 

Comparison of fluorescence-based

quantitation with UV absorbance measurements
 

 

Life Technologies, A Thermo Fisher Scientific Brand